Macroscopic mechanical testing has revealed that chemical segregation at grain boundaries results in a change of the Hall-Petch slope, indicating a change in grain boundary strengthening due to the presence of solute atoms. The project will study the role of chemical segregation on the local yielding of grain boundaries using nano-indentation and in situ transmission electron microscopy to isolate the segregation effect on dislocation-boundary interaction. In the case of segregation induced grain boundary strengthening the experimental results will be considered within a macroscopic framework that the grain boundary energy increases with plastic strain using the atomistic hypothesis that the initial grain boundary deformation causes a disruption of the lowest energy solute atom configuration.